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Ghost Dye™ Blue 516. Ghost Dye™ Red 710. Ghost Dye™ Red 780. Ghost Dye™ UV 450 ... Pacific Blue. Pacific Orange. PE. PE-Alexa Fluor® 610. PE-Alexa Fluor® 700. PE ...Fluorochrome chart FL 450/50 530/30 585/40 630/30 670/40 720/40 780/60 RB Waeeth 3 400 450 500 550 600 650 700 750 800 850 R-PE-Cy7 Cy7 APC-Cy7 AF700 APC-Cy5.5 er DRAQ5 er AF647 R-PE-Cy5.5 R-PE-Cy5 Cy5 APC 7-AAD TR AF594 R-PE-TR PI AF568 R-PE AF555 Cy3 FITC AF488 GFP PB BV421 AF405 DAPI ee uorescet chae n lue chnnel …The net charge on a dextran depends on the fluorophore and the method of preparation. Molecular Probes prepares most of its dextrans by reacting a water-soluble amino dextran with the ... LysoSensor™ Yellow/Blue† 335, 381 452, 521 Indo‡ 356, 343 466, 408 Fura‡ 364, 340 503, 495 Fluo-4 494 516 Oregon ®Green 488 BAPTA-1 496 524 Calcium ...See full list on thermofisher.com The amine-reactive Pacific Blue™ succinimidyl ester can be used to can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ∼410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. View all Pacific Blue™ dye products.. View the Fluorophore Selection Guide. For Research Use Only.Labeling efficiency was a major consideration in the evaluation of our violet dyes, and comparisons have either been reported between V450 and Pacific Blue or presented between V550 and Pacific Orange (Fig. 3), demonstrating that, for certain antibodies, higher numbers of V450 or V550 dye molecules can be attached compared with the number of ...Fluorochrome selection is a key step in designing multi-color antibody panels. The list of available fluorochromes is continuously growing, fitting current needs in clinical flow cytometry to simultaneously use more markers to better define multiple leukocyte subpopulations in a single tube. Several criteria guide fluorochrome selection: …Two of the most commonly used fluorophores in flow cytometry, R-phycoerythrin (R-PE) and allophycocyanin (APC), are phycobiliproteins originally derived from algae. Both proteins contain fluorescent phycobilin chromophores embedded into a protein scaffolding which accounts for their large size.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ... Table of Fluorochromes. This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. Using Pacific Blue and Pacific Orange dyes simultaneously allows you to excite both dyes at the same wavelength while their emissions are differentially distinguished. Pacific Orange and Pacific Blue antibody labeling kits allow the labeling of small amounts of antibody with a violet light-excitable dye (up to 100 µg of antibody per reaction). Fluorescence of different substances under UV light. Green is a fluorescein, red is Rhodamine B, yellow is Rhodamine 6G, blue is quinine, purple is a mixture of quinine and rhodamine 6g. Solutions are about 0.001% concentration in water. Fluorophore molecules could be either utilized alone, or serve as a fluorescent motif of a functional system.Blue. BD Horizon V450. VPD450. Pacific Blue. ™. 375. Blue. Hoechst 33342. Alexa Fluor® 488. Yellow. PE. PI. Orange. PE-Texas Red®. Red. 7-AAD. PE-Cy5. PerCP.Common fluorophores used with laser; Ultraviolet (UV) 355 nm: DAPI, Hoechst, LIVE/DEAD Blue, Brilliant Ultraviolet: Violet: 405–407 nm: Pacific Blue, eFluor 450, Pacific Orange, eFluor 506, Super Bright 436, Super Bright 600, Brilliant Violet, LIVE/DEAD Yellow, LIVE/DEAD Aqua, LIVE/DEAD Violet, CFP : Blue: 488 nmFixable Blue DAPI SYTOX™ Blue Hoechst 33342 ... CO121612 MP Fluorophore Flow Poster Subject: CO121612, MP, Fluorophore, Flow, Poster Created Date: 7/19/2016 1:32:18 PM ...c Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, ... In this work, a blue fluorophore 5-oxopyrrolidine-3-carboxylic acid (1) has been fully isolated from a citric acid and urea CD synthesis and characterized with NMR, electrospray ionization-mass spectrometry (ESI-MS), and Fourier-transform ...DAPI (4′,6-diamidino-2-phenylindole) DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and ...The Alexa Fluor®, Pacific BlueTM, and Pacific OrangeTM Antibody Labeling Kits provide a convenient means to label small amounts of antibodies with the superior Alexa Fluor® dyes or the 405-nm excitable Pacific BlueTM and Pacific OrangeTM dyes. These kits are optimized for labeling 100 μg of antibody per reaction.After staining a cell population with Pacific Blue™, annexin V and SYTOX™ AADvanced™, apoptotic cells show violet fluorescence, dead cells show red fluorescence, and live cells show little or no fluorescence. These populations are easily distinguished by a flow cytometer with the 405 nm and 488 nm lines for excitation.Pacific Green dye is a bright green-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 411/510 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 ...16 авг. 2005 г. ... If calculations indicate that the protein conjugate is labeled with significantly more than seven moles of fluorophore per mole of IgG antibody, ...Getting around town can be a hassle, espefrom that of commonly used green fluorophores. Alexa Flu Pacific Blue™ 410: 455: 450/50: 1: The Fluorophore Brightness Index Score is a relative indication of fluorescence intensity above the background for each fluorophore antibody conjugation (1=dim, 5=brightest). These values can differ depending upon the flow cytometer, the instrument filters and settings, laser power, the antibody clone and ...Abstract. Taxoids such as paclitaxel (Taxol) are an important class of anticancer drugs that bind β-tubulin and stabilize cellular microtubules. To provide new chemical tools for studies of microtubules, we synthesized derivatives of paclitaxel modified at the 7-position with the small coumarin-derived fluorophore Pacific Blue (PB). We would like to show you a description her Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... Pacific Orange dye is a bright orange-fluoresce

In vivo, is an accessory photosynthetic pigment found in blue-green algae: APC-Cy7: Allophycocyanin – Cy7: Tandem conjugate where APC is coupled to a cyanine dye (Cy7) Longest wavelength emission in the family of fluorophores excited by the red laser: A488: Alexa Fluor 488: Incredibly stable, so very popular dye: Spectrum almost identical to ...Pacific Blue™ is part of the BD violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 404-nm and an emission maximum (Em Max) at 455-nm. Pacific Blue is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter).... fluorophores (e.g. Pacific Blue) with highly expressed markers. Spread from a bright fluorophore will mask low level fluorescence in nearby channels.The fluorophore standard beads were first run and detector gains adjusted to put the bead signals near the top third of the log scale (e.g., MFI of 1,000 on a scale of 1-1 10,000). The MFI for the standard beads in the detector channels used for FITC, PE, APC, and Pacific Blue were recorded.

See the characteristics of Pacific Blue dye and the range of antibody conjugates, kits, and other products related to this fluorophore.Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates, as evidenced by over 19,000 citations in the ...…

Reader Q&A - also see RECOMMENDED ARTICLES & FAQs. Not for use in diagnostic or therapeutic pr. Possible cause: Labeling efficiency was a major consideration in the evaluation of our violet dyes, .